Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00116527-BIOTANGBIOLOGICAL-BUFFERS.JPG-250.jpg)
Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm
MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm.
Biotang Inc RNAse Free Phosphate-Buffered Saline, 250 ml
RNAse Free Phosphate-Buffered Saline, 250 ml.
Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only
UNITED STATES BIOLOGICAL CORPORATION DULBECCO MEM W/O GLU PWD 10L
NC2682569 DULBECCO MEM W/O GLU PWD 10L
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-100.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-500.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
![Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10400-1000.0.JPG-250.jpg)
Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
PBS 1X Solution (Phosphate-Buffered Saline) is a balanced, isotonic buffer commonly used in biological and biomedical research. Prepared at a working concentration, it contains sodium chloride, sodium phosphate, and potassium phosphate to maintain pH and osmolarity. It is widely used in cell culture, molecular biology, and immunohistochemistry for cell washing, reagent preparation, and sample dilution. PBS helps preserve the integrity and viability of biological samples by providing a stable, non-disruptive environment. Its compatibility with most biological systems makes it a fundamental laboratory reagent.
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10900-36.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 4.5 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 10 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 4.5ml of PBS per tube
Sterile
36 x 4.5 ml
Abcam Antigen Retrieval Buffer (100 x Tris Buffer, pH 10.0), 125ML
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
The product is subject to the following: Abcam Restricted Use Statement